Sci 250 week 1 staining lab and quiz

SCI250 Week 1 Chapter 3 Staining Lab Quiz  (6.7 points each)

Check or underline the correct answer

Section: Multiple Choice

 

1.     Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids?

  • Gram stain
  • Schaeffer-Fulton stain
  • Acid-fast stain
  • Lipid stain
  • Spore Stain

2.     Which of the following stains is used to classify microorganisms based on their cell wall content?

  • Capsular stain
  • Gram stain
  • Spore stain
  • Negative stain
  • Methylene blue

3.     In the Gram stain, Gram-positive bacteria stain with a _________ color due to the incorporation of the _________ dye into their cell wall.

  • purple, safranin
  • purple, crystal violet
  • red, safranin
  • red, crystal violet

4.     What is the function of the mordant iodine in a Gram staining procedure?

  • A mordant causes the crystal violet to run.
  • A mordant fixes the bacteria to the slide.
  • A mordant keeps dye attached to an object.
  • All of the above

5.     What is the order of reagents used in the Gram stain?

  • crystal violet, iodine, safranin, alcohol
  • alcohol, crystal violet, iodine, safranin
  • iodine, crystal violet, safranin, alcohol
  • crystal violet, iodine, alcohol, safranin
  • crystal violet, safranin, alcohol, iodine

6.     The presence of a capsule around bacterial cells usually indicates their increased disease-causing potential and resistance to disinfection. Capsules are generally viewed by ________

  • spore staining.
  • scanning electron microscopy.
  • Gram staining
  • Negative stain

Section: True or False

 

7.     Fluorescent microscopes use an ultraviolet (UV) light source and fluorochromes as stains.

8.     Older, pure cultures of Gram-positive organisms can appear red when Gram stained.

Section: Matching

 

9.     Use of two or more dyes to differentiate among bacterial species or to distinguish various structures of an organism; for example, the Gram stain.

  • Differential stain
  • Flagellar stain
  • Gram stain
  • Negative stain
  • Schaeffer-Fulton spore stain
  • Simple stain
  • Ziehl-Neelsen acid-fast stain
  • Cationic dye (also called basic dye)

10.  A technique for observing flagella by coating the surfaces of flagella with a dye or a metal such as silver.

  • Differential stain
  • Flagellar stain
  • Gram stain
  • Negative stain
  • Schaeffer-Fulton spore stain
  • Simple stain
  • Ziehl-Neelsen acid-fast stai
  • Cationic dye (also called basic dye)

11.  Technique of staining the background around a specimen, leaving the specimen clear and unstained.

  • Differential stain
  • Flagellar stain
  • Gram stain
  • Negative stain
  • Schaeffer-Fulton spore stain
  • Simple stain
  • Ziehl-Neelsen acid-fast stain
  • Cationic dye (also called basic dye)

12.  A differential stain used to make endospores easier to visualize.

  • Differential stain
  • Flagellar stain
  • Gram stain
  • Negative stain
  • Schaeffer-Fulton spore stain
  • Simple stain
  • Ziehl-Neelsen acid-fast stain
  • Cationic dye (also called basic dye)

13.  A single dye used to reveal basic cell shapes and arrangements.

  • Differential stain
  • Flagellar stain
  • Gram stain
  • Negative stain
  • Schaeffer-Fulton spore stain
  • Simple stain
  • Ziehl-Neelsen acid-fast stain
  • Cationic dye (also called basic dye)

14.  A differential stain for organisms that are not decolorized in alcohol, such as the bacteria that cause Hansen’s Disease (leprosy) and tuberculosis.

  • Differential stain
  • Flagellar stain
  • Gram stain
  • Negative stain
  • Schaeffer-Fulton spore stain
  • Simple stain
  • Ziehl-Neelsen acid-fast stain
  • Cationic dye (also called basic dye)

15.  An ionic compound, used for staining bacteria, in which the positive ion imparts the color.

  • Differential stain
  • Flagellar stain
  • Gram stain
  • Negative stain
  • Schaeffer-Fulton spore stain
  • Simple stain
  • Ziehl-Neelsen acid-fast stain
  • Cationic dye (also called basic dye)

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